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QUANTITATIVE AMINO ACID ANALYSIS Aurélie Lolia Applications Manager, Biochrom Ltd 2 QUANTITATIVE AMINO ACID ANALYSIS ã Principles of amino acid analysis ã Ion exchange chromatography ã The Biochrom 30 physiological system ã Optimisation of chromatography ã Principles ã Separation of less common amino acids ã Troubleshooting and maintenance 3 PRINCIPLES OF AMINO ACID ANALYSIS ã Structure of amino acids ã Where - NH2 is the amino group - COOH is the carboxyl group - R is the side chain ã Separa
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  QUANTITATIVE AMINO ACID ANALYSIS Aurélie Lolia Applications Manager, Biochrom Ltd  2 QUANTITATIVE AMINO ACID ANALYSIS ã Principles of amino acid analysis ãIon exchange chromatographyãThe Biochrom 30 physiological system ã Optimisation of chromatography ãPrinciplesãSeparation of less common amino acids ã Troubleshooting and maintenance  3 PRINCIPLES OF AMINO ACID ANALYSIS ã Structure of amino acidsãWhere-NH2 is the amino group-COOH is the carboxyl group-R is the side chain ã Separation is effected by:ãCharge difference on the amino acids caused by different pKvalues of the side chainsãHydrophobic interaction of the side chain with the polystyrene matrix  4 ION EXCHANGE PROCESS Principle:The positively chargedamino acids are bound to the resin which is negatively charged. The conditions are then altered to increase the pH , temperature and the concentration of the buffer counter ion. When the isoionicpoint of an amino acid is being reached, the ionic attraction to the resin is lost and the amino acid elutes from the column.
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