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Laboratory

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  1. Observations - After adding drops of 1% ammonium thiocyanate solution, the color becomes red that is caused by the formation of FeSCN +2, and it indicates that there is an iron in the blood. 2. Write an ironic equation illustrating the reaction that took place. -  Fe 3+ (aq) + SCN -  (aq) <- -> FeSCN 2+ (aq) (Reddish brown) 3. Why was the nitric acid added in this test? - The nitric acid is the one who is responsible for dissolving the iron. Nitric acid will be added to the blood solution in this test for the detection of iron in the blood. The iron in the blood must be placed in acidic solution. This will prevent the iron in the blood ferric oxide. Thus, the FeSCN 2+   can’t achieve when ferric oxide is  added by ammonium thiocyanate. The nitric acid was being added in this test (iron test) in order to identify if it is positive in iron or there is a presence of iron in the sample. B.2 Proteins 1. Description of precipitate - There is no precipitate formed in this experiment. 2. Results of Biuret test - The color is still the same (colorless) after adding Biuret reagent. 3. Conclusion - There is no precipitate formed and the sample is colorless. After adding the Biuret reagent still the solution didn’t change phys ically (colorless; no precipitate). B.3 Sodium and Potassium 1. Results of flame tests:  a. NaCl solution - It has yellow flame  b. Blood (test for Na) - It has yellow flame c. KCl solution - It has yellow flame d. Blood (test for K) - Using a cobalt glass, the flame turned into a violet color then it slowly turned back into a yellow flame. B.4 Calcium and Magnesium 1. Observations - There is no white precipitate and it indicates that there is no presence of calcium and magnesium ions. Moreover, the solution is colorless.
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