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PEX-12-04

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  Name: jgudExercise 12: Serological Testing: Activity 4: Western Blotting Technique Lab ReportPre-lab Quiz ResultsYou scored 75% by answering 3 out of 4 questions correctly.1. Which of the following tests is not correctly matched to what it tests for?You correctly answered: d. These are all correctly matched.2. Gel electrophoresis is used to separate proteins on the basis of You correctly answered: e. size and charge3. Western blotting can be used to test forYou correctly answered: d. HIV and Lyme disease4. In this activity you will use Western blotting to test for the presence ofYour answer : a. antigens (like the indirect ELISA).Correct answer: b. antibodies (like the indirect ELISA).01/30/19 page 1  01/30/19 page 2Experiment ResultsStop & Think Questions:What is present on the nitrocellulose strips?You correctly answered: a. HIV antigens separated by electrophoresisHow would you describe the binding site of the secondary antibody?You correctly answered: c. the constant region of the primary antibodyExperiment Data: gp160gp120p55p31p24HIV Test ResultPatient AnononononoNegativePatient BnonoyesnoyesIndeterminate Patient CyesyesyesnoyesPositivePositive ControlyesyesyesyesyesPositiveNegative ControlnononononoNegative  01/30/19 page 3Post-lab Quiz ResultsYou scored 75% by answering 3 out of 4 questions correctly.1. The patient samples being tested containYou correctly answered: b. serum.2. The primary antibodies in the Western blotYour answer: a. are bound to the nitrocellulose.Correct answer: c. come from the patient sample.3. A key difference between the ELISA and the Western blot technique is that theYou correctly answered: a. Western blot looks at separated antigens.4. The antibodies in a positive patient sample bind to theYou correctly answered: d. constant region of the enzyme-linked antibody and the antigen bound to the nitrocellulose strip.  01/30/19 page 4Review Sheet Results1. Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV.You did not answer this question.2. Explain the procedure for a patient with an indeterminate HIV Western blot result.You did not answer this question.3. Briefly describe how the nitrocellulose strips were prepared before reacting them with the patient samples.You did not answer this question.4. Describe the importance of the washing steps in the procedure.You did not answer this question.
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