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     #    9    8    0   7     S   t   o   r   e   a   t  –    2    0    °    C Paclitaxel rev. 07/13/09 n  1 mg Background: Paclitaxel belongs to the taxane family of antitumor and antileukemic agents (3). By binding to β -tubulin and promoting the assembly of microtubules, paclitaxel prevents microtubual depolymerization and blocks normal cell division (1–3). The microtubule dysfunction induced by paclitaxel results in aberrant cell mitosis and sometimes apoptosis. The IC 50  of paclitaxel-induced mitotic block is 4 nM (4). Molecular Formula: C 47 H 51 NO 14 ·H 2 O Molecular Weight: 853.92 Da Directions for Use: Paclitaxel is supplied as 1 mg pow-der. Store at or below -20ºC. Before use, dissolve powder in 1.15 ml DMSO to make a 1 mM paclitaxel stock solution. For working concentrations of 100 nM-1000 nM, dilute DMSO stock 1:10,000 to 1:1000. Treat cells with the desired concentration for 6-48 hours. Paclitaxel is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC. Background References:  (1) Rowinsky, E.K. et al. (1990) J. Natl. Cancer Inst.  82, 1247–1259. (2) Jordan, A. et al. (1998) Med. Res. Rev.  18, 259–296. (3) Abal, M. et al. (2003) Curr. Cancer Drug Targets   3, 193–203. (4) Long, B.H. and Fairchild, C.R. (1994) Cancer Res.  54, 4355–4361. Storage: Store tightly sealed at –20°C. Please visit for a complete listing of recommended companion products. Orders  n 877-616-CELL (2355) Support  n 877-678-TECH (8324) Web  n     ©     2    0    0   7    C   e    l    l    S    i   g   n   a    l    i   n   g    T   e   c    h   n   o    l   o   g   y ,    I   n   c . kDa Phospho-cdc25C(Thr48)cdc25C Paclitaxel (µM)200140100806050200140100806050           0 1          0  .          1          0  .          0          1          0  .          0          0          1 Western blot analysis of extracts from HT29 cells, either un- treated or treated with indicated concentration of paclitaxel using Phospho-Cdc25C (Thr48) Antibody #9527 (upper) or control Cdc25C (5H9) Rabbit mAb Antibody #4688 (lower).   3 H 5 C 6 H 5 C 6 C 6 H 5 NHOHOHHOO OOOOOO OOOO Species Cross-Reactivity Key:H —human M —mouse R —rat Hm —hamster Mk —monkey Mi —mink C —chicken Dm —D. melanogaster X —Xenopus Z —zebrafish B —bovine Dg —dog Pg —pig Sc —S. cerevisiae All —all species expectedSpecies enclosed in parentheses are predicted to react based on 100% homology. Applications Key:W —Western IP —Immunoprecipitation IHC —Immunohistochemistry ChIP —Chromatin Immunoprecipitation IF —Immunofluorescence F —Flow cytometry E-P —ELISA-Peptide This product is for in vitro   research use only and is not intended for use in humans or animals. This product is not intended for use as a therapeutic or in diagnostic procedures.     #    9    8    0   7    M    S    D    S Orders n  877-616-CELL (2355) Support n   877-678-TECH (8324) Web n     ©     2    0    0    8    C   e    l    l    S    i   g   n   a    l    i   n   g    T   e   c    h   n   o    l   o   g   y ,    I   n   c . Material Safety Data Sheet (MSDS) for Paclitaxel I. Identication: Product name:  Paclitaxel Product Catalog:  9807 CAS number:  Not applicable to mixtures Manufacturer Supplier:  Cell Signaling Technology 3 Trask Lane Danvers, MA 01923 USA 978-867-2300 TEL 978-867-2400 FAX 978-578-6737 EMERGENCY TEL II. Composition/Information:III. Hazard Identication: To the best of our knowledge, the chemical, physical, and toxicological properties of this solu-tion have not been thoroughly investigated. RTECS: DA8340700 EMERGENCY OVERVIEW: May be an irritant. May cause irritation to eyes, skin, mucous membranes. HMIS rating:  Health 1 Flammability 0 Reactivity 0 IV. First Aid Measures: Inhalation:  If inhaled, remove to fresh air. If breathing is difficult, get medical attention. Ingestion:  If swallowed, wash out mouth with water provided person is conscious. Get medical attention. Skin exposure:  In case of contact, immediately wash skin with soap and water for at least 15 minutes. Remove contaminated clothing. Wash clothing before reuse. Eye exposure:  In case of contact with eyes, immediately flush eyes with water for at least 15 minutes. Get medical attention. V. Fire Fighting Measures: Flash Point: Data not available.  Autoignition Temperature: Data not available.  Fire extinguishing media: Water spray, dry chemical, foam, or carbon dioxide. Firefighting: Wear protective clothing and self-contained breathing apparatus to prevent contact with skin and eyes.   VI. Accidental Release Measures: Take appropriate precautions to minimize direct contact with skin, eyes, mucous membranes, and prevent inhalation of dust. Isolate the spill area. Wear approved self-contained breathing apparatus, protective clothing and chemical resistant gloves. Sweep up carefully to avoid raising dust and transfer to a chemical waste container for disposal according to local, state, and federal regulations. Ventilate area and wash spill site after material pickup is complete. Do not let substance enter drains. VII. Handling And Storage: Storage:  Store in tightly closed container at -20°C. Avoid contact with skin, eyes, mucous membranes and respiratory system. Wash hands thoroughly after handling. VIII. Exposure Controls/Personal : Occupational exposure limits not found. Ventilation System:  A system of local and/or general exhaust is recommended. Skin Protection:  Wear compatible chemical resistant gloves and protective clothing. Eye protection:  Wear protective safety glasses. Maintain eye wash fountain and quick-drench facilities in work area. IX. Physical And Chemical PropertiesX. Stability and Reactivity: Stability: Stable under recommended storage conditions.  Conditions to avoid: Moisture. Protect from light and heat.  Materials to avoid: Avoid acids and bases.  Hazardous Decomposition: Carbon oxides, sulfur oxides.  Hazardous exothermic reactions: During fire conditions may produce toxic gases such as carbon monoxide, carbon dioxide. XI. Toxicological Information:  To the best of our knowledge, the chemical, physical, and toxicological properties of this solution have not been thoroughly investigated. RTECS # DA8340700 Acute Effects:  Data not available. Primary Effects:  Data not available. May be an irritant to eyes, skin, respiratory system. Chronic Effects:  Data not available. XII. Ecological Information: Data not available for this product. XIII. Disposal Considerations: Dispose of in accordance with federal, state, local environmental regulations. XIV. Transport Information: DOT: Proper Shipping Name: None.This substance is considered non-hazardous for transport.  IATA: Proper Shipping Name: None.This substance is considered non-hazardous for air transport. XV. Regulatory Information: EU Regulations/Classifications/Labeling Information:  Not available for this product. US Regulatory Information: Not SARA listed. XVI. Other Information: This product is not intended for use in humans. To the best of our knowledge, this document is accurate. It is intended to serve as a guide for safe use of this product in a laboratory setting by experienced personnel. The burden of safe use of this material rests entirely with the user. The above information is believed to be accurate but is not necessarily all-inclusive and shall be used only as a guide. Cell Signaling Technology, Inc., shall not be held liable for any damage resulting from the handling of or from contact with the above product. Substance Name: Paclitaxel Synonyms: Taxol CAS#: 33069-62-4 Molecular Weight: 853.92 Molecular Formula: C 47 H 51 NO 14 Appearance: crystalline powder Color: white Odor: none Boiling Point: data not available Melting Point: 210-220°C Ignition temperature data not available Volatile Organic Compounds data not available pH data not available Solubility: not soluble in water; soluble in DMSO, alcohols, and chlorinated solvents
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