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Beginning Molecular Biology Laboratory Manual-UMBC.2003 [Por Trisquelion]

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Molecular Biology Laboratory Manual Beginning Molecular Biology Laboratory Manual Department of Biological Sciences CHAPTER 1: General Laboratory Methods Safety Procedures Preparation of Solutions Disposal of Buffers and Chemicals Equipment Micropipets Using a pH meter Autoclave operating procedures Operating instructions for spectrophotometer Working with DNA Sterile Technique CHAPTER 2: Instructions for Notebook Keeping CHAPTER 3: Computer User's Guide UNIX commands File Transfer The World
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   Molecular Biology Laboratory Manual Beginning Molecular Biology Laboratory Manual Department of Biological Sciences   CHAPTER 1: General Laboratory Methods Safety Procedures Preparation of Solutions Disposal of Buffers and Chemicals Equipment Micropipets Using a pH meter Autoclave operating procedures Operating instructions for spectrophotometer Working with DNA Sterile Technique CHAPTER 2: Instructions for Notebook KeepingCHAPTER 3: Computer User's Guide UNIX commandsFile Transfer The World Wide Web The Wisconsin Package - gcg. http://www.research.umbc.edu/~jwolf/method1.htm (1 of 3)22/03/2005 11:25:28   Molecular Biology Laboratory Manual CHAPTER 4: Molecular Biology Methods Common Stock SolutionsM.1: Preparation of genomic DNA from bacteria M.2: PCR amplification of DNA M.3: Restriction enzyme digestion of DNA M.4: Phenol/chloroform extraction of DNA M.5: Ethanol precipitation of DNA M.6: Agarose gel electrophoresis M.7: Transformation of E. coli   by electroporation M.8: Wizard PCR preps DNA purification system M.9: Alternate method for purifying DNA from agarose gels M.10: Transfection of mammalian cells using Lipofectamine (LTI) M.11: Southern BlottingM.12:Western Blot Analysis of Epitoped-tagged Proteins for alkaline phosphatase conjugated antibodies M.13: Preparation of sequencing gels M.14: Isolation of RNA from mammalian cells using RNAZOL (Teltest)  CHAPTER 5: Tissue Culture Methods Types of cells grown in culture Work area and equipment Preservation and storage Maintenance Safety considerations Tissue culture methods Determining cell counts http://www.research.umbc.edu/~jwolf/method1.htm (2 of 3)22/03/2005 11:25:28   Molecular Biology Laboratory Manual This Web page is maintained by  Julie B. Wolf  , UMBC;Last updated on12/08/2003 http://www.research.umbc.edu/~jwolf/method1.htm (3 of 3)22/03/2005 11:25:28  Applied Molecular Biology Applied Molecular Biology at UMBC  Department of Biological Sciences  UMBC's 9-month Master's Degree program in Applied Molecular Biology (AMB) is designed for students interested in careers in industrial and biomedical science. For more information on the program and for application information, follow the link at the left. Laboratory Instructor: Julie B. Wolf  Useful links: Laboratory Courses: Biol. 635L and Biol 636L. The core of the Applied Molecular Biology Program at UMBC is a two-semester laboratory course in which the students work on independent research projects in collaboration with UMBC research faculty. These courses are only open to students in the 9-month Master's Degree Program.Projects for 2003-2004 1. Cloning, expression, and binding site determination of the C. elegans transcription factor, egl-18.   2. Optimization of codon usage for expression of the human NKX3.1 gene in E. coli   using gene assembly PCR. 3. Cloning, expression and functional analysis of the human RGR protein in COS cells. 4. Comparative sequence analysis of the catsup gene in sister Drosophila species. 5. Construction of mutant and epitope-tagged variants of the nitA gene from Volvox   for use in transformation. 6. Construction of MHC Class II-expressing and her2 antigen-expressing retroviral constructs for use in breast cancer vaccine research 7. Development of methods for detecting SNP's in genomic samples of bird species using real-time PCR 8. Determination of   ddc   expression levels in Drosophila melanogaster   in response to wounding 9. Random mutagenesis of hexokinase genes Looking for a Job? This Web page is maintained by  Julie B. Wolf  , UMBC;Last updated on 12/02/2003 http://www.research.umbc.edu/~jwolf/index.htm22/03/2005 11:25:33
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