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Maldi Tof ms Lipids

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Maldi TOF lipidios
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             Khalid A. Al-Saad 1 , Vladimir Zabrouskov 2 , William F. Siems 1 , N. Richard Knowles 2 ,Richard M. Hannan 3 and Herbert H. Hill Jr. 1 * 1 Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA 2 Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA 99164-4630, USA 3 USDA-ARS Regional Plant Introduction Station, Washington State University, Pullman, WA 99164-4630, USA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              *                                                                                                                                                                                                                                                    Table 1.  Lipid ions observed in MALDI spectra (non-N-containing lipids)                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           Figure 1.  Positive ion MALDI spectra of (a) TAG(12:0/14:0/ 14:0) and (b) TAG(14:0/14:0/16:0). (c) Relative intensities offragment ions due to the loss of carboxylate group ‘B’ inTAGs(A/A/B), where the signal intensities due to the loss ofcarboxylate groups ‘A’ are normalized to 100%. The measuredintensities for each TAG were averages of 10 spectra. The barsrepresent fragment ions produced from: 1. TAG(12:0/12:0/ 14:0); 2. TAG(14:0/14:0/12:0); 3. TAG(14:0/14:0/16:0); 4.TAG(16:0/16:0/14:0); 5. TAG(18:0/18:0/14:0); and 6. TAG(18:0/ 18:0/16:0). The MALDI plate wells each contained approx.50ng of a TAG. The relative intensities of the prompt fragmentsare consistent with the relative abundances of the fatty acylgroups in the neutral TAGs.                                                                                                                                                                                                                                                                                                                                                                                                                               Figure 2.  (a) Positive ion MALDI spectrum of TAG(14:0/14:0/ 14:0). (b) PSD-MALDI spectrum of TAG(14:0/14:0/14:0); theprecursor ion was [TAG  Na]  . Scheme1.  Proposed fragmentation pathways of TAG. The solid arrow (a) shows the pathway leadingto the prompt fragment ions and the dotted arrows (b, c) show the pathways leading to fragmentsobserved in the PSD experiments. Both sn-1 and sn-2 substituents can also be expelled followingsimilar pathways.                          
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