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Report Bacteria

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  1.0OBJECTIVE The bacteriological quality of water sample is measured by performing total platecount. 2.0THEORY Bacteria are the highest population of microorganisma in a wastewater treatment plant will belong to the bacteria. They are single-celled organismswhich use soluble food. Conditions in the treatment plant are adjusted so thatchemoherotrophs predominate. Bacteria are highly adaptable to diverseenvironmental conditions.Understanding of bacteria and their metabolic processes has beenexpanded vastly. There are more bacteria as separate individuals than any other type of organism there can be as many as !. billion bacteria in one gram of fertile soil. #n bacterial colony assays the patterns are formed within culturemedia that has been inoculated with  bacterial cells. This allows the cells toreproduce and form bacterial colonies within and$or on the surface of the medium.%hen the colonies are sufficiently large they are usually visible to the na&ed eyewhich allows researchers to determine the number of colonies formed. #naddition various visual characteristics of the colonies such as shape si'e pigmentation and opacity can be used to help determine the type of bacterium present. (ost experiment requires the quantitative determination of bacterial populations. The two typical methods for determining bacterial numbers are thestandard or viable plate count method and spectrophotometric )turbidimetric*analysis. +lthough the two methods yield the similar results there are distinctdifferences. The standard plate count method consists of diluting a sample withsterile saline or phosphate buffer diluent until the bacteria are dilute enough tocount accurately. The assumption is that each viable bacterial cell is separate fromall others and will develop into a single discrete colony )C,U*.  3.0MATERIAL .etri plate!.ipette /.Test tube0.1lass rod .Bunsen burner 2.#ncubator 3.4thanol 5 6 7 methanol8.9terili'er 5.(icroscope:.Bacteria medium;- eptone < g-Beef 4xtract < /g-+gar <  g-=istilled water < 2::m>  4.0PROCEDUREProcedures o !re! r#$% $u&r#e$& 'ed# . g of peptone /g of beef extract and  g of agar are mixed in 2::m> distilledwater and boiled.!.+fter boiling the agar is left cooled to 0 - : o C. D#(u&#o$ .:.m> of the bacteria sample is blown into a test tube )Tube ?* contained of 5.5m> of dilution fluid )distilled water* by using a cleaned sterile dried pipette.Continue blowing bubbles for a second or two for good mixing!.:.m> sample from Tube ? is blown into Tube ?! continue blowing bubbles for a second or two for good mixing./.+nother :.m> from of sample from Tube ?! is pipette and blown into Tube ?/continue blowing bubbles for a second or two for mixing.0.The same procedures are repeated until Tube ?2. .>abel your tubes with the dilution factor as to notice the bacteria content in thetubes.  )!re d P( &e &es& 'e&*od .,or the 9pread late test the nutrient media is poured into half of the six petri plates.!.:.m> of diluted sample from each test tube is sprin&led into six different petri plates contain sterile agar using a stic&./.The petri plates are then closed and labeled.0.+ll the petri plates are placed inside the incubator for !0 hours with atemperature of /3 o C. Pour P( &e &es& 'e&*od .:.m> of diluted sample from each test tube is sprin&led into six different petri plates contain sterile agar using a stic&.!.The agar is poured into the plates and till the agar solidified./.The petri plates are then closed and labeled.0.+ll the petri plates are placed inside the incubator for !0 hours with atemperature of /3 o C. Me&*ods o cou$&#$% + c&er# .The petri plates are ta&en out after being incubated for !0 hours.!.The selected petri plate is placed on the counting chamber./.The bacteria colonies on the culture is counted and recorded.
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