Restriction enzymes

restriction enzymes and specific DNA cleavage
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  Restriction Enzymes and Site- Specific DNA Cleavage   a class of enzymes known as restriction endonucleases or restriction enzymes are able to cleave DNA molecules at the  positions at which particular, short sequences of bases are  present. For example, the enzyme  BamHI recognizes the double-stranded  sequence 5'- GGATCC-3' 3'-CCTA GG-5'    Mechanism of DNA cleavage by the restriction enzyme BamHI. The enzyme makes a single cut in the backbone of each DNA strand wherever the duplex contains a BamHI restriction site    The nucleotide sequence recognized for cleavage by a restriction enzyme is called the restriction site of the enzyme  The restriction enzymes cleave their restriction site asymmetrically (at different sites in the two DNA strands), but some restriction enzymes cleave symmetrically (at the same site in both strands). The former leave sticky ends because each end of the cleaved site has a small, single-stranded overhang that is complementary in base sequence to the other end.
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