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  2D NMR Spectroscopy:COSY Jessica Thomaston  Outline 1. History2. Theory of 2D NMR3. Applications of COSY  A brief history of 2D NMR ! In 1971, the idea of 2D NMR was proposed by Jean Jeener. ! The 1975 Ernst paper ÒTwo-dimensional spectroscopy,application to nuclear magnetic resonanceÓ utilized JeenerÕsidea to produce spectra. ! 1980s-present: application of NMR to protein structures ! In 1991 Ernst won a Nobel Prize inChemistry for his contributions toFourier Transform NMR  Types of 2D NMR ! COSY: correlation spectroscopy ! HETCOR: heteronuclear COSY ! TOCSY: total correlation spectroscopy ! NOESY: Nuclear Overhauser effectspectroscopy ! EXSY: exchange spectroscopy ! J-Spectroscopy  Experimental ! A series of equally-spaced t 1  are used; 90û pulses occur atwhite circles ! Each spectrum measures the instantaneous state of theperturbed system after various t 1
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