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Identification of molecular marker associated with mungbean yellow mosaic virus resistance in mungbean [Vigna radiata (L.) Wilczek

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Mungbean [Vigna radiata (L.) Wilczek] is an important legume which can be grown in varying environmental conditions, during all three crop seasons viz., kharif, rabi and spring/summer in India, as sole or inter crop for grain and green manure. It is
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  Vol.:(0123456789)  1 3 Vegetos https://doi.org/10.1007/s42535-019-00063-y RESEARCH ARTICLES Identification of molecular marker associated with mungbean yellow mosaic virus resistance in mungbean [ Vigna radiata  (L.) Wilczek] Darshan T. Dharajiya 1,2  · Y. Ravindrababu 3 Received: 26 January 2019 / Revised: 24 September 2019 / Accepted: 26 September 2019 © Society for Plant Research 2019 Abstract Mungbean [ Vigna radiata  (L.) Wilczek] is an important legume which can be grown in varying environmental conditions, during all three crop seasons viz., kharif, rabi  and spring/summer in India, as sole or inter crop for grain and green manure. It is an excellent source of easily digestible proteins with low flatulence, which complements the staple rice diet in Asia. Mungbean Yellow Mosaic Disease (MYMD) caused by whitefly (  Bemisia tabaci ) transmitted mungbean Yellow Mosaic Virus (MYMV) is an important constraint of mungbean. To fulfill future demands, there is a need to use molecular marker technology and other biotechnological interventions. Hence, Bulked Segregant Analysis (BSA) with a Random Amplified Polymorphic DNA (RAPD) marker technique was used to analyze the F 2:3  individuals of Pusa Ratna (susceptible) × Meha (resistant) to identify the molecular marker linked to MYMV resistance in mungbean. Field screening of thirty-five mungbean genotypes against MYMV was carried out, prior to crossing program to identify the resistant and susceptible genotypes. The RAPD primer, OPP 07 showed the specific band of 895 bp in resistant parent and their bulks, but not in the susceptible parent and their bulks. Co-segregation analysis was performed in resistant and susceptible F 2:3  individuals; it confirmed that OPP 07 895  marker was associated with MYMV resistance in mungbean. The linked RAPD molecular marker OPP 07 895 , can be used for the identification of (Quantitative Trait Locus) QTL for MYMV resistance and marker assisted selection program in mungbean. Keywords   Vigna radiata  (L.) Wilczek · Bulked segregant analysis · Molecular marker · RAPD · Mungbean yellow mosaic virus (MYMV) Introduction Mungbean [ Vigna radiata  (L.) Wilczek] is one of the most important, widely distributed pulse crop in India as well as South Asia. It is a self-pollinated diploid plant having 2 n  = 2  x  = 22 chromosomes with a genome size of 0.60 pg/1C (579 Mb/1C) belongs to family leguminosae  and sub-family  papilionaceae  (Somta and Srinives 2007; Sehrawat et al. 2014). In the traditional vegetarian diet of Indian popula-tion, pulses occupy second place next to cereals. Mungbean is an excellent source of easily digestible proteins with low flatulence, which complements the staple rice diet in Asia (Khattak et al. 2002; Saminathan 2013). Mungbean was cultivated in about 3.38 million hectares during 2013–14 with a total production of 1.61 million tones and a productivity of 474 kg/ha in India (Anonymous 2015). The yield of mungbean has been stagnant over the years. Improvement in yield of mungbean is becoming dif-ficult, mainly due to the occurrence of pest and diseases (Karthikeyan et al. 2012). Major biological stresses of mungbean are mungbean yellow mosaic virus, leaf crinkle virus, anthracnose, powdery mildew, Cercospora  leaf spot, gram pod borer, bruchid and whitefly (Pande et al. 2000). Plant viral diseases cause serious economic losses in many major crops by reducing seed yield and quality (Kang et al. 2005). Among the various diseases, the mungbean yellow *  Darshan T. Dharajiya darshanbiotech1@gmail.com 1  Department of Plant Molecular Biology and Biotechnology, C. P. College of Agriculture (CPCA), Sardarkrushinagar Dantiwada Agricultural University (SDAU), Sardarkrushinagar, Palanpur, Gujarat 385506, India 2  Department of Biotechnology, Mehsana Urban Institute of Sciences, Ganpat University, Ganpat Vidyanagar, Mahesana, Gujarat 384012, India 3  Pulses Research Station, SDAU, Sardarkrushinagar, Palanpur, Gujarat 385506, India  Vegetos  1 3 resistance in mungbean. This can be used for the develop-ment of MYMV resistance mungbean genotypes using MAS in breeding programs. Acknowledgements  Authors are grateful to the authorities of Pulses Research Station and C. P. College of Agriculture, SDAU, Sardarkrush-inagar, India, for providing facilities to conduct the research work. References Anonymous (2015) E-Pulses data book. Area, production and yield of different Pulses in India. Indian Institute of Pulses Research (IIPR). Kanpur, India. http://www.iipr.res.in/e-pulse -data-book.html. 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